Molecular and Cellular Mechanisms of Lipid Droplet Breakdown in the Liver of Chinese Soft-Shelled Turtle (Pelodiscus sinensis)

The Chinese soft-shelled turtle ( Pelodiscus sinensis ) is among the most primitive amphibians and reptiles in nature. On account of its environmental suitability unique hibernation habit, peculiar physiological phenomenon P. attracted attention researchers field marine science. present study aimed to investigate molecular mechanisms underlying periodic variation lipid droplet (LD) liver . Histological results indicated that accumulated LD during non-hibernation was gradually consumed hibernation. RNA-Seq revealed genes responsible for carbohydrate catabolism were down-regulated hibernation, while involved oxidation up-regulated. These suggest energy metabolism changes i.e., generation mode shifted from oxidation. Further analysis both lipolysis autophagy could promote degradation hepatic To further determine relationship between process breakdown, we applied inhibitors (diethylumbelliferyl phosphate 3-Methyladenine) cultured primary hepatocytes main way hepatocyte data provide clear evidence about seasonal hepatocytes, corresponding with different